PDF | The present study aims to evaluate the therapeutic properties of the medicinal plants (Vitex negundo, Aegle marmelos and Boswellia serrata) for their . Abstract To evaluate the anti-inflammatory activity of Vitex negundo (VN) leaf extract and its mechanism of action in experimental animals. Carrageenin induced. Abstract. The present study was undertaken to assess the anti-inflammatory effect of ethanolic extract of Vitex negundo roots in rats.

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The plant material was collected from Versova, Mumbai and plant sample was authenticated from Blatter Herbarium, St.

Albino rats of either sex were divided into five groups of six animals each. The carrageenan-induced paw edema in inflamamtory is believed to be biphasic.

Plasma MDA Malondialdehyde estimation: The leaves were dried under shade and were ground to form the smooth powder. Footnotes Kulkarni, et al.: Group I received distilled water and served as control. Kundnani College of Pharmacy.

Antioxidant and Antiinflammatory Activity of Vitex negundo.

DX 7 days, orally as standard drug. International Journal of Pharmacology Volume 2 3: The experiment was repeated for ten wnti. The oxidative stress is the condition where Reactive Oxygen Species ROS generation exceeds endogenous anti-oxidant defense Sen, and it is well known that in chronic and sub-acute inflammation reactive oxygen species play an important role in modulating the extent of inflammatory response and consequent tissue and cell injury Robbin et al.

Oxytocin was dissolved in distilled water to get a desired concentration. Leaves are applied externally to bruises and eruption [ 9 ]. The present study indicated VN to possess significant anti-inflammatory activity in dose dependent manner against acute as well as sub-acute inflammatory experimental models. The plant material and preparation of extract: International Journal of Pharmacology, 2: Herbal drugs play an important role in health care programs especially in developing countries.


Effect of VN on oxidative stress in sub-acute inflammatory model. Effects of Luffa echinata on lipid peroxidation and free Radical scavenging Activity. Oxytocin Sandoz and oestradiol valerate Sigma organon were also used for the present study. It belongs to the family Verbanaceae [ 4 ]. The reactive oxygen species are also known to activate matrix metalloproteinase e. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

The reduced level of MDA was taken as indicator of anti-lipoperoxidative activity, which can be taken as index of reduced oxidative stress. Pharmacological Experiment on Isolated Preparations. It was identified and authenticated by an expert botanist from J.

Curcumin was used as positive control.

These observations suggest that VN possesses anti-inflammatory activity against acute as well as sub-acute inflammation, which appear to be due to prostaglandin inhibition and reduction of oxidative stress respectively, which needs to be substantiated by further study. C Harinath, Director and Head J.

Antioxidant and Antiinflammatory Activity of Vitex negundo.

Abstract Reactive oxygen species are implicated in various inflammatory disorders. The fresh leaves of VN were cleaned of extraneous matter, shade dried and powdered.

During the aerobic incubation of tissue homogenates, malondialdehydes are formed which on reaction with thiobarbituric acid produced a pink colour. The mechanism of anti-inflammatory action was explored by observing its effect on oxytocin induced contractions in rat uterus and oxidative stress. Steam distillate of the leaves can be used as stomachic, purgative, febrifuge and antiemetic [ 8 ]. Similarly, fresh leaves of VN have been suggested to possess anti-inflammatory and pain suppressing activities possibly mediated via PG synthesis inhibition, antihistamine, membrane stabilising and antioxidant activities Dharmasiri et al.


Statistical analysis was carried out by applying student’s t-test.

The basic literature survey exposed that Vitex negundo hasantioxidant, antiulcer and anti-nociceptive activity and Murraya koenigii possessing anthelmintic, analgesic, cooling activity, but not many scientific studies have been carried out to reveal and collaborate the anti-inflammatory activity of Vitex negundo and Murraya koenigii leaves.

Dose selection of test drug was based on previously reported studies Telang et al. Anti-inflammatory Drugs from Plants and Marine sources. Moreover, leaves of VN are known to possess various anti-oxidant chemical constituents like flavonoids Banerji et al. For mechanism of action: A positive lipid peroxidation inhibitory activity of the extract of V. Stabilization of the Prussian blue colour in the determination of polyphenols.

The anti-inflammatory activity in this dose was also comparable with Ibuprofen mg kg -1 B. Screening of anti-inflammatory activity of certain indigenous drugs on carrageenin induced hind paw oedema in rats.

It is also useful in leucoderma and blood disorders. Pergamon Press, New York, pp: This is because of the adverse effects associated with synthetic drugs. All the respective grouped animals were injected with carrageenan 0. Gum acacia, phosphoric acid, potassium ferricyanide, ferric chloride were of LR grade and obtained from local suppliers.

Edema in one of the paws of the rat was induced by injecting into plantar aponeurosis 0. The effects of VN in dose concentration ranging from 0. X 7 days orally and Fitex mg kg -1 B. Springer Science and Business Media, J Agri Food Chem